Review



abi 3130 genetic analyzer  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier
Bioz Manufacturer Symbol Thermo Fisher manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99

    Structured Review

    Thermo Fisher abi 3130 genetic analyzer
    Abi 3130 Genetic Analyzer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/abi+3130+genetic+analyzer/Forma+Water+Jacketed+CO2+Incubator/pmc13101558-64-17-21
    Average 99 stars, based on 1 article reviews
    abi 3130 genetic analyzer - by Bioz Stars, 2026-10
    99/100 stars

    Images

    Related Articles

    other:

    Article Title: Intratumoral fungal burden of Candida tropicalis as a novel prognostic biomarker for recurrence and mortality in colorectal cancer.
    Article Snippet: The microsatellite instability analysis was evaluated using five mononucleotide repeat markers, which were BAT25, BAT26, D2S123, D5S346, and D17S250, by an ABI 3130 Genetic Analyzer (Applied Biosystems), as described previously.36,37

    Article Title: Intratumoral fungal burden of Candida tropicalis as a novel prognostic biomarker for recurrence and mortality in colorectal cancer
    Article Snippet: The microsatellite instability analysis was evaluated using five mononucleotide repeat markers, which were BAT25, BAT26, D2S123, D5S346, and D17S250, by an ABI 3130 Genetic Analyzer (Applied Biosystems), as described previously.,

    In Vivo:

    Article Title: Functional Analysis of MysERG1 , a Novel Immune-Related Gene in Encapsulation Regulation, in the Oriental Armyworm Mythimna separata (Lepidoptera: Noctuidae)
    Article Snippet: First-strand cDNA was synthesized from 100 ng of total RNA with Oligo (dt) using SuperScript IV Reverse Transcriptase (Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturer’s instructions. .. Using cDNA from in vivo encapsulation samples as a template and the primers listed in , the complete MysERG1 coding sequence was amplified by PCR and verified on an ABI 3130 Genetic Analyzer (Applied Biosystems, Foster City, CA, USA). ..

    Article Title: Functional Analysis of MysERG1, a Novel Immune-Related Gene in Encapsulation Regulation, in the Oriental Armyworm Mythimna separata (Lepidoptera: Noctuidae)
    Article Snippet: First-strand cDNA was synthesized from 100 ng of total RNA with Oligo (dt)18 using SuperScript IV Reverse Transcriptase (Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturer’s instructions. .. Using cDNA from in vivo encapsulation samples as a template and the primers listed in Table 1, the complete MysERG1 coding sequence was amplified by PCR and verified on an ABI 3130 Genetic Analyzer (Applied Biosystems, Foster City, CA, USA). ..

    Encapsulation:

    Article Title: Functional Analysis of MysERG1 , a Novel Immune-Related Gene in Encapsulation Regulation, in the Oriental Armyworm Mythimna separata (Lepidoptera: Noctuidae)
    Article Snippet: First-strand cDNA was synthesized from 100 ng of total RNA with Oligo (dt) using SuperScript IV Reverse Transcriptase (Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturer’s instructions. .. Using cDNA from in vivo encapsulation samples as a template and the primers listed in , the complete MysERG1 coding sequence was amplified by PCR and verified on an ABI 3130 Genetic Analyzer (Applied Biosystems, Foster City, CA, USA). ..

    Article Title: Functional Analysis of MysERG1, a Novel Immune-Related Gene in Encapsulation Regulation, in the Oriental Armyworm Mythimna separata (Lepidoptera: Noctuidae)
    Article Snippet: First-strand cDNA was synthesized from 100 ng of total RNA with Oligo (dt)18 using SuperScript IV Reverse Transcriptase (Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturer’s instructions. .. Using cDNA from in vivo encapsulation samples as a template and the primers listed in Table 1, the complete MysERG1 coding sequence was amplified by PCR and verified on an ABI 3130 Genetic Analyzer (Applied Biosystems, Foster City, CA, USA). ..

    Sequencing:

    Article Title: Functional Analysis of MysERG1 , a Novel Immune-Related Gene in Encapsulation Regulation, in the Oriental Armyworm Mythimna separata (Lepidoptera: Noctuidae)
    Article Snippet: First-strand cDNA was synthesized from 100 ng of total RNA with Oligo (dt) using SuperScript IV Reverse Transcriptase (Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturer’s instructions. .. Using cDNA from in vivo encapsulation samples as a template and the primers listed in , the complete MysERG1 coding sequence was amplified by PCR and verified on an ABI 3130 Genetic Analyzer (Applied Biosystems, Foster City, CA, USA). ..

    Article Title: Functional Analysis of MysERG1, a Novel Immune-Related Gene in Encapsulation Regulation, in the Oriental Armyworm Mythimna separata (Lepidoptera: Noctuidae)
    Article Snippet: First-strand cDNA was synthesized from 100 ng of total RNA with Oligo (dt)18 using SuperScript IV Reverse Transcriptase (Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturer’s instructions. .. Using cDNA from in vivo encapsulation samples as a template and the primers listed in Table 1, the complete MysERG1 coding sequence was amplified by PCR and verified on an ABI 3130 Genetic Analyzer (Applied Biosystems, Foster City, CA, USA). ..

    Article Title: Updated morphological description and molecular analysis of Neoechinorhynchus ( Neoechinorhynchus ) iraqensis (Acanthocephala: Neoechinorhynchidae) from Planiliza abu (Heckel) (Mugilidae) in Iraq
    Article Snippet: PCR reactions (25 μl) consisted of 5 μl 1 mM deoxyribonucleotide triphosphates (dNTPs, Biotools, Spain), 0.80 μl of each primer, 2.5 μl of 10× Taq buffer (Biotools) with MgCl2, 0.70 μl of Taq polymerase (1 U; Biotools) and 12.20 μl of distilled water. .. PCR cycling comprised denaturation at 94 °C for 1 min, followed by 35 cycles of 94 °C for 1 min, annealing at 56 °C for 1 min, and extension at 72 °C for 1 min, followed by a terminal extension at 72 °C for 10 min. Sequencing reactions were electro-phoresed, and sequencing was performed using the BigDye Terminator v3.1 Cycle Sequencing Kit on an ABI 3130 Genetic Analyzer (Applied Biosystems, Foster City, California) according to the manufacturer’s protocol. .. Contigs were amassed and base-calling dissimilarities resolved using Codoncode Aligner version 3.5.4 (Codoncode Corporation, Dedham, Massachusetts).

    Amplification:

    Article Title: Functional Analysis of MysERG1 , a Novel Immune-Related Gene in Encapsulation Regulation, in the Oriental Armyworm Mythimna separata (Lepidoptera: Noctuidae)
    Article Snippet: First-strand cDNA was synthesized from 100 ng of total RNA with Oligo (dt) using SuperScript IV Reverse Transcriptase (Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturer’s instructions. .. Using cDNA from in vivo encapsulation samples as a template and the primers listed in , the complete MysERG1 coding sequence was amplified by PCR and verified on an ABI 3130 Genetic Analyzer (Applied Biosystems, Foster City, CA, USA). ..

    Article Title: Functional Analysis of MysERG1, a Novel Immune-Related Gene in Encapsulation Regulation, in the Oriental Armyworm Mythimna separata (Lepidoptera: Noctuidae)
    Article Snippet: First-strand cDNA was synthesized from 100 ng of total RNA with Oligo (dt)18 using SuperScript IV Reverse Transcriptase (Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturer’s instructions. .. Using cDNA from in vivo encapsulation samples as a template and the primers listed in Table 1, the complete MysERG1 coding sequence was amplified by PCR and verified on an ABI 3130 Genetic Analyzer (Applied Biosystems, Foster City, CA, USA). ..

    Polymerase Chain Reaction:

    Article Title: Functional Analysis of MysERG1 , a Novel Immune-Related Gene in Encapsulation Regulation, in the Oriental Armyworm Mythimna separata (Lepidoptera: Noctuidae)
    Article Snippet: First-strand cDNA was synthesized from 100 ng of total RNA with Oligo (dt) using SuperScript IV Reverse Transcriptase (Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturer’s instructions. .. Using cDNA from in vivo encapsulation samples as a template and the primers listed in , the complete MysERG1 coding sequence was amplified by PCR and verified on an ABI 3130 Genetic Analyzer (Applied Biosystems, Foster City, CA, USA). ..

    Article Title: Functional Analysis of MysERG1, a Novel Immune-Related Gene in Encapsulation Regulation, in the Oriental Armyworm Mythimna separata (Lepidoptera: Noctuidae)
    Article Snippet: First-strand cDNA was synthesized from 100 ng of total RNA with Oligo (dt)18 using SuperScript IV Reverse Transcriptase (Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturer’s instructions. .. Using cDNA from in vivo encapsulation samples as a template and the primers listed in Table 1, the complete MysERG1 coding sequence was amplified by PCR and verified on an ABI 3130 Genetic Analyzer (Applied Biosystems, Foster City, CA, USA). ..

    Article Title: Characterization of a novel inherited splice-site variant in MAPK8IP3 expands the genetic and phenotypic spectrum of neurodevelopmental disorders
    Article Snippet: PCR amplifications were performed in a reaction volume of 25 μL, which included 2 units of Taq DNA polymerase (Takara Biotechnology, Dalian, China). .. Subsequently, the purified PCR products were sequenced bidirectionally using both forward and reverse primers on an ABI 3130 Genetic Analyzer (Applied Biosystems, CA, USA). .. Total RNA was isolated from peripheral blood samples obtained from the proband, two family members (mother, sister), and three unrelated healthy controls, using the RNAprep Pure High Efficiency Total RNA Extraction Kit (TIANGEN, Beijing, China).

    Article Title: Updated morphological description and molecular analysis of Neoechinorhynchus ( Neoechinorhynchus ) iraqensis (Acanthocephala: Neoechinorhynchidae) from Planiliza abu (Heckel) (Mugilidae) in Iraq
    Article Snippet: PCR reactions (25 μl) consisted of 5 μl 1 mM deoxyribonucleotide triphosphates (dNTPs, Biotools, Spain), 0.80 μl of each primer, 2.5 μl of 10× Taq buffer (Biotools) with MgCl2, 0.70 μl of Taq polymerase (1 U; Biotools) and 12.20 μl of distilled water. .. PCR cycling comprised denaturation at 94 °C for 1 min, followed by 35 cycles of 94 °C for 1 min, annealing at 56 °C for 1 min, and extension at 72 °C for 1 min, followed by a terminal extension at 72 °C for 10 min. Sequencing reactions were electro-phoresed, and sequencing was performed using the BigDye Terminator v3.1 Cycle Sequencing Kit on an ABI 3130 Genetic Analyzer (Applied Biosystems, Foster City, California) according to the manufacturer’s protocol. .. Contigs were amassed and base-calling dissimilarities resolved using Codoncode Aligner version 3.5.4 (Codoncode Corporation, Dedham, Massachusetts).

    Article Title: Characterization of a novel inherited splice-site variant in MAPK8IP3 expands the genetic and phenotypic spectrum of neurodevelopmental disorders
    Article Snippet: The resulting cDNA was amplified by PCR using LA Taq DNA polymerase (Takara, Beijing, China) with the following gene-specific primers: forward primer 5′–CCAGTTCACCGTCTGCAAC–3' (E20F) and reverse primer 5′–CTGCTGCTGTCAGGCTCTG–3' (E23R). .. The PCR products were resolved by electrophoresis on a 2.5% agarose gel and subsequently sequenced using an ABI 3130 genetic analyzer (Applied Biosystems, USA). .. The amplified cDNA fragments were purified and cloned into the pEASY-T5 Zero Cloning Vector (TransGen Biotech, Beijing, China) via TA cloning.

    Purification:

    Article Title: Characterization of a novel inherited splice-site variant in MAPK8IP3 expands the genetic and phenotypic spectrum of neurodevelopmental disorders
    Article Snippet: PCR amplifications were performed in a reaction volume of 25 μL, which included 2 units of Taq DNA polymerase (Takara Biotechnology, Dalian, China). .. Subsequently, the purified PCR products were sequenced bidirectionally using both forward and reverse primers on an ABI 3130 Genetic Analyzer (Applied Biosystems, CA, USA). .. Total RNA was isolated from peripheral blood samples obtained from the proband, two family members (mother, sister), and three unrelated healthy controls, using the RNAprep Pure High Efficiency Total RNA Extraction Kit (TIANGEN, Beijing, China).

    Electrophoresis:

    Article Title: Characterization of a novel inherited splice-site variant in MAPK8IP3 expands the genetic and phenotypic spectrum of neurodevelopmental disorders
    Article Snippet: The resulting cDNA was amplified by PCR using LA Taq DNA polymerase (Takara, Beijing, China) with the following gene-specific primers: forward primer 5′–CCAGTTCACCGTCTGCAAC–3' (E20F) and reverse primer 5′–CTGCTGCTGTCAGGCTCTG–3' (E23R). .. The PCR products were resolved by electrophoresis on a 2.5% agarose gel and subsequently sequenced using an ABI 3130 genetic analyzer (Applied Biosystems, USA). .. The amplified cDNA fragments were purified and cloned into the pEASY-T5 Zero Cloning Vector (TransGen Biotech, Beijing, China) via TA cloning.

    Agarose Gel Electrophoresis:

    Article Title: Characterization of a novel inherited splice-site variant in MAPK8IP3 expands the genetic and phenotypic spectrum of neurodevelopmental disorders
    Article Snippet: The resulting cDNA was amplified by PCR using LA Taq DNA polymerase (Takara, Beijing, China) with the following gene-specific primers: forward primer 5′–CCAGTTCACCGTCTGCAAC–3' (E20F) and reverse primer 5′–CTGCTGCTGTCAGGCTCTG–3' (E23R). .. The PCR products were resolved by electrophoresis on a 2.5% agarose gel and subsequently sequenced using an ABI 3130 genetic analyzer (Applied Biosystems, USA). .. The amplified cDNA fragments were purified and cloned into the pEASY-T5 Zero Cloning Vector (TransGen Biotech, Beijing, China) via TA cloning.



    Similar Products

    99
    Thermo Fisher abi 3130 genetic analyzer
    Abi 3130 Genetic Analyzer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/abi+3130+genetic+analyzer/Forma+Water+Jacketed+CO2+Incubator/pmc13101558-64-17-21
    Average 99 stars, based on 1 article reviews
    abi 3130 genetic analyzer - by Bioz Stars, 2026-10
    99/100 stars
      Buy from Supplier

    Image Search Results